a new approach for designing a potentially vaccine candidate against urinary tract infection by using protein display on lactobacillus

b ackground: the prevalence of urinary tract infection (uti) is really high in the world. escherichia coli is a major agent of uti. one of the strategies for decreasing uti infections is vaccine development. as the attachment is a really important stage in colonization and infection, at- tachment inhibition has an applied strategy.  fimh protein is a major factor during bacterial colonization in urinary tract and could be used as a vaccine. thus, it was considered in this research as a candidate anti- gen. methods: the sequences of fimh and acma genes were used for de- signing a synthetic gene. it was cloned to pet23a expression vector and transformed  to e. coli (de3) origami.  to confirm the expression  of recombinant  protein,  sds-page  and western  blotting  methods  were used.  subsequently,  recombinant  protein  was  purified.  on  the  other hand, lactobacillus reuteri was cultured and mixed with fimh / acma recombinant  protein. the rate of protein localization  on lactobacillus surface was assessed using elisa method. r esults: it was showed that the recombinant protein was expressed in e. coli (de3) origami and purified by affinity chromatography. more- over, this protein could be localized on lactobacillus surface by 5 days. conclusion:   in current study,  a fusion recombinant  protein was pre- pared and displayed on l. reuteri surface. this strain could be used for animal  experiment  as  a  competitor  against  uropathogenic   e.  coli (upec). using manipulated probiotics strains instead of antibiotic ther- apy could decrease the antibiotic consumption  and reduce multi-drug resistant strains.